Chemical gradient-mediated melting curve analysis for genotyping of SNPs
نویسندگان
چکیده
منابع مشابه
Chemical-mediated Melting Curve Analysis for Genotyping of Single Nucleotide Polymorphisms
This report describes a new microfluidic solid-phase chemical-mediated melting curve analysis method for Single Nucleotide Polymorphism (SNP) genotyping. The method is based on allele-specific denaturation of DNA duplexes upon exposure to dynamic chemical gradient. DNA duplexes conjugated on beads are captured in a microfluidic gradient generator device. The device is designed with dams, keepin...
متن کاملMelting curve analysis of SNPs (McSNP): a gel-free and inexpensive approach for SNP genotyping.
High-throughput methods for assaying DNA variation require two important steps: (i) discriminating the variation and (ii) detecting the signal. In this report, we describe a novel SNP genotyping method that we refer to as melting curve analysis of SNPs (McSNP). McSNP combines a classic approach for discriminating alleles, restriction enzyme digestion, with a more recent method for detecting DNA...
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Instrumentation, chemistry, and software for high-throughput genotyping using fluorescent melting curves are described. The LightTyper system provides post-amplification genotyping within 10 min using samples in 96- or 384-well microplate formats. The system is homogenous because all reagents are added at the beginning of the reaction and there is no sample manipulation between amplification an...
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25 A single-tube method – ligation mediated real-time PCR high-resolution melt analysis 26 (LMqPCR HRMA) – was modified for rapid typing of ESKAPE-pathogens. A 97 % 27 agreement (60/62 isolates) was achieved in comparison to PFGE which indicates that 28 LMqPCR HRMA is a rapid and accurate screening tool to monitor for nosocomial outbreaks. 29 on O cber 1, 2017 by gest ht://jcm .sm .rg/ D ow nla...
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Real-time monitoring of PCR is one of the most important methods for DNA and RNA detection widely used in research and medical diagnostics. Here we describe a new approach for combined real-time PCR monitoring and melting curve analysis using a 3' end-blocked Exciton-Controlled Hybridization-sensitive fluorescent Oligonucleotide (ECHO) called Eprobe. Eprobes contain two dye moieties attached to...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2009
ISSN: 0173-0835
DOI: 10.1002/elps.200800729